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prism 3130 genetic analyzer capillary dna sequencer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher prism 3130 genetic analyzer capillary dna sequencer
    Prism 3130 Genetic Analyzer Capillary Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+3130+genetic+analyzer/Forma+Water+Jacketed+CO2+Incubator/pmc13099216-79-52-59
    Average 99 stars, based on 1 article reviews
    prism 3130 genetic analyzer capillary dna sequencer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Loss of LLGL1 Elevates EGFR/RAS/MAPK Signaling and Remodels EMT Markers in Huh-7 Hepatocellular Carcinoma Cells.
    Article Snippet: .. gRNA-encoding sequences cloned into PX462 vectors and genomic LLGL1 loci from selected LLGL1 KO and SC CRISPR/Cas9n Huh-7 clones were analyzed by Sanger DNA sequencing using an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). .. Prior to Sanger sequencing, genomic DNA from Huh-7 clones was isolated using the NucleoSpin DNA Isolation kit (Cat. No: 740952; MachereyNagel, Düren, Germany).

    Article Title: Loss of LLGL1 Elevates EGFR/RAS/MAPK Signaling and Remodels EMT Markers in Huh-7 Hepatocellular Carcinoma Cells
    Article Snippet: .. gRNA-encoding sequences cloned into PX462 vectors and genomic LLGL1 loci from selected LLGL1 KO and SC CRISPR/Cas9n Huh-7 clones were analyzed by Sanger DNA sequencing using an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). .. Prior to Sanger sequencing, genomic DNA from Huh-7 clones was isolated using the NucleoSpin DNA Isolation kit (Cat. No: 740952; Macherey-Nagel, Düren, Germany).

    CRISPR:

    Article Title: Loss of LLGL1 Elevates EGFR/RAS/MAPK Signaling and Remodels EMT Markers in Huh-7 Hepatocellular Carcinoma Cells.
    Article Snippet: .. gRNA-encoding sequences cloned into PX462 vectors and genomic LLGL1 loci from selected LLGL1 KO and SC CRISPR/Cas9n Huh-7 clones were analyzed by Sanger DNA sequencing using an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). .. Prior to Sanger sequencing, genomic DNA from Huh-7 clones was isolated using the NucleoSpin DNA Isolation kit (Cat. No: 740952; MachereyNagel, Düren, Germany).

    Article Title: Loss of LLGL1 Elevates EGFR/RAS/MAPK Signaling and Remodels EMT Markers in Huh-7 Hepatocellular Carcinoma Cells
    Article Snippet: .. gRNA-encoding sequences cloned into PX462 vectors and genomic LLGL1 loci from selected LLGL1 KO and SC CRISPR/Cas9n Huh-7 clones were analyzed by Sanger DNA sequencing using an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). .. Prior to Sanger sequencing, genomic DNA from Huh-7 clones was isolated using the NucleoSpin DNA Isolation kit (Cat. No: 740952; Macherey-Nagel, Düren, Germany).

    DNA Sequencing:

    Article Title: Loss of LLGL1 Elevates EGFR/RAS/MAPK Signaling and Remodels EMT Markers in Huh-7 Hepatocellular Carcinoma Cells.
    Article Snippet: .. gRNA-encoding sequences cloned into PX462 vectors and genomic LLGL1 loci from selected LLGL1 KO and SC CRISPR/Cas9n Huh-7 clones were analyzed by Sanger DNA sequencing using an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). .. Prior to Sanger sequencing, genomic DNA from Huh-7 clones was isolated using the NucleoSpin DNA Isolation kit (Cat. No: 740952; MachereyNagel, Düren, Germany).

    Article Title: Loss of LLGL1 Elevates EGFR/RAS/MAPK Signaling and Remodels EMT Markers in Huh-7 Hepatocellular Carcinoma Cells
    Article Snippet: .. gRNA-encoding sequences cloned into PX462 vectors and genomic LLGL1 loci from selected LLGL1 KO and SC CRISPR/Cas9n Huh-7 clones were analyzed by Sanger DNA sequencing using an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). .. Prior to Sanger sequencing, genomic DNA from Huh-7 clones was isolated using the NucleoSpin DNA Isolation kit (Cat. No: 740952; Macherey-Nagel, Düren, Germany).

    Article Title: Phenotype-Driven Next-Generation Sequencing and Structure-Based In Silico Analysis Reveal Disease-Specific Diagnostic Yield and Genotype-Phenotype Correlations in Inherited Kidney Diseases.
    Article Snippet: As integrated in Franklin and Genomize platforms, DANN, MutationTaster, PredictSNP2SIFT, SIFT4G, Provean, Mutation assessor, FATHMM, FATHMM-MKL, FATHMM-XF, MetaSVM, MetaLR, EIGEN, EIGEN PC, LRT, DEOGEN2, https://doi.org/10.3390/life16030500 MVP, MutPred, REVEL, PrimateAI, and SpliceAI, Human Splicing Finder were used to predict the pathogenicity of variants [12,16]. .. Confirmation of the identified variant and the segregation within the family was performed using Sanger sequencing on an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems, Thermo Fisher Scientific, Tokyo, Japan). ..

    Marker:

    Article Title: Local Genetic Diversity and Structure of Asian Black Bear, Ursus thibetanus, Populations in Nagano, Japan
    Article Snippet: Human–bear conflict is a significant issue in Japan.. Understanding genetic diversity, population structure, and local population size of the Asian black bear is essential for improving ecological knowledge and developing conservation and management measures of this species.. We examined sex, mitochondrial (mt) DNA variations, and 12 nuclear microsatellite genotypes from hair-derived DNA collected in the Kiso region, Nagano Prefecture.

    Software:

    Article Title: Local Genetic Diversity and Structure of Asian Black Bear, Ursus thibetanus, Populations in Nagano, Japan
    Article Snippet: Human–bear conflict is a significant issue in Japan.. Understanding genetic diversity, population structure, and local population size of the Asian black bear is essential for improving ecological knowledge and developing conservation and management measures of this species.. We examined sex, mitochondrial (mt) DNA variations, and 12 nuclear microsatellite genotypes from hair-derived DNA collected in the Kiso region, Nagano Prefecture.

    Variant Assay:

    Article Title: Phenotype-Driven Next-Generation Sequencing and Structure-Based In Silico Analysis Reveal Disease-Specific Diagnostic Yield and Genotype–Phenotype Correlations in Inherited Kidney Diseases
    Article Snippet: As integrated in Franklin and Genomize platforms, DANN, MutationTaster, PredictSNP2SIFT, SIFT4G, Provean, Mutation assessor, FATHMM, FATHMM-MKL, FATHMM-XF, MetaSVM, MetaLR, EIGEN, EIGEN PC, LRT, DEOGEN2, MVP, MutPred, REVEL, PrimateAI, and SpliceAI, Human Splicing Finder were used to predict the pathogenicity of variants [ , ]. .. Confirmation of the identified variant and the segregation within the family was performed using Sanger sequencing on an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems, Thermo Fisher Scientific, Tokyo, Japan). ..

    Article Title: Phenotype-Driven Next-Generation Sequencing and Structure-Based In Silico Analysis Reveal Disease-Specific Diagnostic Yield and Genotype-Phenotype Correlations in Inherited Kidney Diseases.
    Article Snippet: As integrated in Franklin and Genomize platforms, DANN, MutationTaster, PredictSNP2SIFT, SIFT4G, Provean, Mutation assessor, FATHMM, FATHMM-MKL, FATHMM-XF, MetaSVM, MetaLR, EIGEN, EIGEN PC, LRT, DEOGEN2, https://doi.org/10.3390/life16030500 MVP, MutPred, REVEL, PrimateAI, and SpliceAI, Human Splicing Finder were used to predict the pathogenicity of variants [12,16]. .. Confirmation of the identified variant and the segregation within the family was performed using Sanger sequencing on an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems, Thermo Fisher Scientific, Tokyo, Japan). ..

    Sequencing:

    Article Title: Phenotype-Driven Next-Generation Sequencing and Structure-Based In Silico Analysis Reveal Disease-Specific Diagnostic Yield and Genotype–Phenotype Correlations in Inherited Kidney Diseases
    Article Snippet: As integrated in Franklin and Genomize platforms, DANN, MutationTaster, PredictSNP2SIFT, SIFT4G, Provean, Mutation assessor, FATHMM, FATHMM-MKL, FATHMM-XF, MetaSVM, MetaLR, EIGEN, EIGEN PC, LRT, DEOGEN2, MVP, MutPred, REVEL, PrimateAI, and SpliceAI, Human Splicing Finder were used to predict the pathogenicity of variants [ , ]. .. Confirmation of the identified variant and the segregation within the family was performed using Sanger sequencing on an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems, Thermo Fisher Scientific, Tokyo, Japan). ..

    Article Title: Molecular Detection and Phylogenetic Analysis of Selected Viral Pathogens in Wild Boar Populations of Russia.
    Article Snippet: .. Sanger sequencing was performed in both directions with the previously mentioned PCR primers using the Big Dye 3.1 Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) on the ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. The obtained sequence chromatograms were analyzed, trimmed and assembled into consensus sequences using SeqMan Lasergene 11.1.0 software (DNASTAR, Madison, WI, USA).

    Article Title: Detection and genetic characterization of atypical porcine pestivirus in wild boars in European Russia
    Article Snippet: The PCR products of APPeV-positive samples with 896 bp in size were purified via the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s protocol. .. The purified DNA was sequenced in both directions using the Big Dye 3.1 Terminator Cycle Sequencing Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer’s instructions and carried out on the ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific, Carlsbad, CA, USA) sequencing device. .. The obtained sequences were processed using SeqMan Lasergene 11.1.0 (DNASTAR, Madison, WI, USA).

    Polymerase Chain Reaction:

    Article Title: Molecular Detection and Phylogenetic Analysis of Selected Viral Pathogens in Wild Boar Populations of Russia.
    Article Snippet: .. Sanger sequencing was performed in both directions with the previously mentioned PCR primers using the Big Dye 3.1 Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) on the ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. The obtained sequence chromatograms were analyzed, trimmed and assembled into consensus sequences using SeqMan Lasergene 11.1.0 software (DNASTAR, Madison, WI, USA).

    Electrophoresis:

    Article Title: Multimodal analysis of cell-free DNA identifies epigenetic biomarkers for amyotrophic lateral sclerosis diagnosis and progression
    Article Snippet: .. Electrophoresis was performed on an ABI PRISM® 3130 Genetic Analyzer (Life Technologies, Foster City, California, USA). .. The data were analyzed using the Peak Scanner software (Applied Biosystems, Waltham, Massachusetts, USA).

    Purification:

    Article Title: Detection and genetic characterization of atypical porcine pestivirus in wild boars in European Russia
    Article Snippet: The PCR products of APPeV-positive samples with 896 bp in size were purified via the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s protocol. .. The purified DNA was sequenced in both directions using the Big Dye 3.1 Terminator Cycle Sequencing Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer’s instructions and carried out on the ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific, Carlsbad, CA, USA) sequencing device. .. The obtained sequences were processed using SeqMan Lasergene 11.1.0 (DNASTAR, Madison, WI, USA).



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